Techniques Used in Microscopy

3D and 4D Electron Tomography

Atomic-Resolution 3D Electron Microscopy with Dynamic Diffraction

Michael A. O'Keefe, Kenneth H. Downing, Hans-Rudolf Wenk, and Hu Meisheng, February 15, 2005. Lawrence Berkeley National Laboratory. Paper LBNL-57522.

Link: Atomic-Resolution 3D Electron Microscopy with Dynamic Diffraction

Three-dimensional reconstruction of single macromolecules

Wadsworth Center, Albany, NY

Link: Three-dimensional reconstruction of single macromolecules

Cameras for Microscopes

CCD Camera Image Correction Methods

Link: CCD Camera Image Correction Methods

Got Good Color?- Color Matching for digital microscopy

Link: Got Good Color?- Color Matching for digital microscopy

Image Capture Methods for Microscope Cameras

This article explains the advantages of three shot color for microscope cameras. From Brian L. Kuyatt and Philip Merlo, Diagnostic Instruments, and Robert Weaver, McCrone Research Institute.

Link: Image Capture Methods for Microscope Cameras

Pixel Array Size Needed to Replace Photomicrographs on Film

Ted Clarke, Scientific Photographer and Instrument Maker

From: McCrone Associates, Inc.
Link: Pixel Array Size Needed to Replace Photomicrographs on Film

Resolution: 3-Shot vs Color Mosaic

Whitepaper from Diagnostic Instruments

Link: Resolution: 3-Shot vs Color Mosaic

Compound Microscopes

Diffracted Light Contrast: Improving the Resolution of a Basic Light Microscope by an Order of Magnitude

A modification of the century-old oblique illumination technique can dramatically improve the resolution of a basic light microscope. The question is raised as to whether the Abbe limit ever truly defined the ultimate resolution of a microscope.

Link: Diffracted Light Contrast: Improving the Resolution of a Basic Light Microscope by an Order of Magnitude

EDX Microanalysis

High Resolution X-ray Spectroscopy close to Room Temperature

Struder L, Meidinger N, Stotter D, Kemmer J, Lechner P, Leutenegger P, Soltau H, Eggert F, Rohde M, Schulein T., MPI fur Extraterrestrische Physik, Halbleiterlabor, Paul-Gerhardt-Allee 42, D-81245 Munich, Germany

From: Bruker Nano GmbH
Link: High Resolution X-ray Spectroscopy close to Room Temperature

Field Ion Microscopy

Field Ion Microscopy and Atom Probe Tomography

Brief description of Field Ion Microscopy and Atom Probe tomography techniques.

Link: Field Ion Microscopy and Atom Probe Tomography

Fundamentals of the Atom Probe

The Three-Dimensional Atom Probe (3DAP) microscope is a point projection microscope that resolves millions of individuals atom positions in three dimensions and identifies the species of each atom by time-of-flight (TOF) mass spectrometry.

From: Imago Scientific Instruments
Link: Fundamentals of the Atom Probe

Fluorescence Multi Photon

FP6 Automation Project

A novel imaging system providing high-content high-throughput multi-dimensional analysis of microscopic biological structure inside non-adherent living cells.

Link: FP6 Automation Project

Green Fluorescent Protein

what is Green Fluorescent Protein and why is it so important.

Link: Green Fluorescent Protein

Handbook of Optical filters for Fluorescence Microscopy

From: Chroma Technology Corp.
Link: Handbook of Optical filters for Fluorescence Microscopy

Optimize Your System with the Right Filter Set

R. Kinoshita, Biophotonics International, Nov. 2002

Link: Optimize Your System with the Right Filter Set

SLM microscopy: scanless two-photon imaging and

Article on two photon imaging microscope that uses a diffractive spatial light modulator (SLM) to shape an incoming two-photon laser beam into any arbitrary light pattern.

Link: SLM microscopy: scanless two-photon imaging and

Total Internal Reflection Fluorescence Microscopy: Introduction and Applications

From: Nikon Instruments USA
Link: Total Internal Reflection Fluorescence Microscopy: Introduction and Applications

Image Software & Automation

FP6 Automation Project

A novel imaging system providing high-content high-throughput multi-dimensional analysis of microscopic biological structure inside non-adherent living cells.

Link: FP6 Automation Project

Micropositioning for Microscopy

An overview of combining submicron positioning stages with resolutions of 20- to 50-nm and repeatability accuracies as low as 200 nm with powerful imaging software to make your microscope more effective.

From: Applied Scientific Instrumentation (ASI)
Link: Micropositioning for Microscopy

Raman FTIR & UV/Vis/NIR

Chemical Imaging without Dyeing

Confocal Raman Microscopy in Bio-Medical and Pharmaceutical Research, Harald Fischer, Andrea Jauss, Imaging & Microscopy (June 05).

Link: Chemical Imaging without Dyeing

Coherent Raman Imaging

Stimulated Raman Scattering microscopy and Coherent Anti-Stoke Raman Scattering (CARS) microscopy are label-free imaging techniques based on vibrational spectroscopy, and are capable of real-time, noninvasive examination of living cells and organisms.

Link: Coherent Raman Imaging

Nondestructive, High-Resolution Materials Characterization with the Confocal Raman-AFM

Link: Nondestructive, High-Resolution Materials Characterization with the Confocal Raman-AFM

Scanning Electron Microscopes

Nanomagnetic Imaging

NIST researchers have developed a modified form of scanning electron microscopy that can reveal the magnetic characteristics of such a surface without disturbing the magnetization.

Link: Nanomagnetic Imaging

Scanning Probe, AFM

Near-field Scanning Optical Microscopy (NSOM)

Link: Near-field Scanning Optical Microscopy (NSOM)

Nondestructive, High-Resolution Materials Characterization with the Confocal Raman-AFM

Link: Nondestructive, High-Resolution Materials Characterization with the Confocal Raman-AFM

Single-molecule recognition imaging microscopy

Describes a technique in an AFM that allows recognition of a specific type of molecule in a complex sample (chromatin) while simultaneously yielding high-resolution topographic images.

Link: Single-molecule recognition imaging microscopy

Tranmission Electron TEM

Introduction to Fluctuation Microscopy

Fluctuation microscopy has been used to study disordered materials including amorphous Ge and silicon. This paper is a general introduction to the technique.

Link: Introduction to Fluctuation Microscopy

Cell and Molecular Biology

High-throughput Vertebrate Screening

a high-throughput platform for cellular resolution

Link: High-throughput Vertebrate Screening

Green Fluorescent Protein

what is Green Fluorescent Protein and why is it so important.

Link: Green Fluorescent Protein

Single-molecule recognition imaging microscopy

Describes a technique in an AFM that allows recognition of a specific type of molecule in a complex sample (chromatin) while simultaneously yielding high-resolution topographic images.

Link: Single-molecule recognition imaging microscopy

Forensics

Hand-Sectioning and Identification of Pressure-Sensitive Tapes

From: McCrone Associates, Inc.
Link: Hand-Sectioning and Identification of Pressure-Sensitive Tapes

Materials

Chemical Imaging without Dyeing

Confocal Raman Microscopy in Bio-Medical and Pharmaceutical Research, Harald Fischer, Andrea Jauss, Imaging & Microscopy (June 05).

Link: Chemical Imaging without Dyeing

Nondestructive, High-Resolution Materials Characterization with the Confocal Raman-AFM

Link: Nondestructive, High-Resolution Materials Characterization with the Confocal Raman-AFM