Optofluidic Microscopy (OFM)

Optofluidic microscopy (OFM) is a new compact and lensless microscopic imaging technique invented in the Biophotonics Laboratory, lead by Professor Changhuei Yang. The device utilizes microfluidic flow to deliver specimens across array(s) of micrometer-size apertures defined on a metal-coated CMOS sensor to generate direct projection images.

Link: Optofluidic Microscopy (OFM)

Innovations in Light Microscopy

Innovations in light microscopes over the past centuries has been driven by scientists who wish to observe and measure phenomena that were smaller, fainter, (Figure 1) and deeper inside tissue than ever before.

From: Nikon Instruments USA
Link: Innovations in Light Microscopy

A New Approach to Fluorescence Microscopy

GE & Science Prize for Young Life Scientists winning essay by Mark Bates describing his discovery of a new type of “optically switchable” fluorescent molecule, and how these molecules were used for high-resolution biological imaging.

Link: A New Approach to Fluorescence Microscopy

Blinded by the Light

Published in 2004, the article reviews advances in light microscopy including 4Pi, STED, confocal imaging, and total internal reflection fluorescence.

Link: Blinded by the Light

Fluorescence microscopy with super-resolved optical sections

Alexander Egner1 and Stefan W. Hell, TRENDS in Cell Biology Vol.15 No.4 April 2005

Link: Fluorescence microscopy with super-resolved optical sections

High Resolution Light Microscopy of Live Cells

Microscopy Today, V. Vodyanoy, June 2005

Link: High Resolution Light Microscopy of Live Cells

New Innovations for Biological Imaging and Instrumentation at HHMI

The Applied Physics and Instrumentation Group (APIG) at Janelia Farms seeks to understand bioimaging challenges and to harness new technologies for advanced imaging. Three initial projects will characterize this effort: higher resolution novel microscopy, high-throughput microscopy, and correlative microscopy.

Link: New Innovations for Biological Imaging and Instrumentation at HHMI

Nonlinear structured-illumination microscopy:

Technical details on structured-illumination microscopy.

Link: Nonlinear structured-illumination microscopy:

Pushing the Envelope in Biological Imaging

Eric Betzig develops novel optical imaging tools in an effort to open new windows into molecular, cellular, and neurobiology.

Link: Pushing the Envelope in Biological Imaging

Sharper Image

An overview of the development of the the Palm

Link: Sharper Image

Shattering the diffraction limit of light: A revolution in fluorescence microscopy?

Link: Shattering the diffraction limit of light: A revolution in fluorescence microscopy?

Single-molecule biology and Bioimaging Zhuang Research Lab

Zhuang Lab at Harvard University is developing real-time fluorescence imaging methods to track the behavior of single virus particles and of single viral genomes in live cells.

Link: Single-molecule biology and Bioimaging Zhuang Research Lab

Squint Busters

October 2006 article on microscopes that are pushing resolution to single-molecule sharpness.

Link: Squint Busters

Super-Resolution Microscopy Captures Molecules in Motion

A new twist on a sophisticated light microscopy technique is enabling researchers to capture short videos of fast-moving cellular processes while delivering super high resolution images of whole cells.

Link: Super-Resolution Microscopy Captures Molecules in Motion

Popular Mechanics:

Mehmet Fatih Yanik and his student, William Putnam, propose a non-invasive electron microscope that won't destroy living cells by using a quantum mechanical measurement technique that allows electrons to sense objects remotely.

Link: Popular Mechanics:

New development in FESEM Technology

The development of the scanning electron microscope and technological improvements to the original design.

From: Carl Zeiss NTS SAS
Link: New development in FESEM Technology

Devising Nano Vision for an Optical Microscope

Phase-sensitive, scatter-field optical imaging is under development at the National Institute of Standards and Technology (NIST). This hybrid version of the optical microscope might be able to image and measure features smaller than 10 nanometers.

Link: Devising Nano Vision for an Optical Microscope

Popular Mechanics:

Mehmet Fatih Yanik and his student, William Putnam, propose a non-invasive electron microscope that won't destroy living cells by using a quantum mechanical measurement technique that allows electrons to sense objects remotely.

Link: Popular Mechanics:

A New Approach to Fluorescence Microscopy

GE & Science Prize for Young Life Scientists winning essay by Mark Bates describing his discovery of a new type of “optically switchable” fluorescent molecule, and how these molecules were used for high-resolution biological imaging.

Link: A New Approach to Fluorescence Microscopy

Super-Resolution Microscopy Captures Molecules in Motion

A new twist on a sophisticated light microscopy technique is enabling researchers to capture short videos of fast-moving cellular processes while delivering super high resolution images of whole cells.

Link: Super-Resolution Microscopy Captures Molecules in Motion

Shattering the diffraction limit of light: A revolution in fluorescence microscopy?

Link: Shattering the diffraction limit of light: A revolution in fluorescence microscopy?