A New Approach to Fluorescence Microscopy

GE & Science Prize for Young Life Scientists winning essay by Mark Bates describing his discovery of a new type of “optically switchable” fluorescent molecule, and how these molecules were used for high-resolution biological imaging.

Link: A New Approach to Fluorescence Microscopy

Popular Mechanics:

Mehmet Fatih Yanik and his student, William Putnam, propose a non-invasive electron microscope that won't destroy living cells by using a quantum mechanical measurement technique that allows electrons to sense objects remotely.

Link: Popular Mechanics:

Shattering the diffraction limit of light: A revolution in fluorescence microscopy?

Link: Shattering the diffraction limit of light: A revolution in fluorescence microscopy?

Super-Resolution Microscopy Captures Molecules in Motion

A new twist on a sophisticated light microscopy technique is enabling researchers to capture short videos of fast-moving cellular processes while delivering super high resolution images of whole cells.

Link: Super-Resolution Microscopy Captures Molecules in Motion

Blackburn Gets Personal, Reflects on Path Leading Up to Nobel Prize

A week after being named UCSF’s—and her native Australia’s—first woman to receive a Nobel Prize in Physiology or Medicine, molecular biologist Elizabeth Blackburn, PhD, sat down with UCSF Chancellor Sue Desmond-Hellmann, MD, MPH, to discuss the elusive goal of work-life balance and the importance of following one’s passions and making time for “intense relaxation.”

Link: Blackburn Gets Personal, Reflects on Path Leading Up to Nobel Prize

Dr. Janet Rowley

In the 1970s, Dr. Janet Rowley identified a specific genetic “translocation” in leukemia, heralding a new understanding of the role of some translocations in specific cancers.

Link: Dr. Janet Rowley

George E. Palade The Nobel Prize in Physiology or Medicine 1974 Autobiography

This autobiography/biography was written at the time of the award and later published in the book series Les Prix Nobel/Nobel Lectures.

Link: George E. Palade The Nobel Prize in Physiology or Medicine 1974 Autobiography

George E. Palade: Legacy of a Distinguished Scientist:

In the early 1950s, Palade’s utilization of the then new technologies, such as electron microscopy and ultracentrifugation,brought understanding of the cell to a new level.

Link: George E. Palade: Legacy of a Distinguished Scientist:

Building a Blueprint of the Cell's Protein Factory

HHMI Bulletin, January 2001

Link: Building a Blueprint of the Cell's Protein Factory

Disease Carriers: Explore the Microscopic World of Health Science

Learn about the science underlying public health issues by perusing -- and using -- digitized images on view.

Link: Disease Carriers: Explore the Microscopic World of Health Science

Mitochondria

Summary of researh using electron tomography to study the invaginations in the mitochondrial inner membran.

Link: Mitochondria

Optical Aspirations

Article on the use of cryo electron microscopy to visualize tiny three-dimensional protein structures inside cells. Nikolaus Grigorieff's lab at HHMI.

Link: Optical Aspirations

The Kinetochore

Summary of research using Electron tomography to assess kinetochore modulation of microtubule dynamics.

Link: The Kinetochore

The mechanism by which chromosomal DNA molecules are held together: entrapment within cohesin rings?

Link: The mechanism by which chromosomal DNA molecules are held together: entrapment within cohesin rings?

The Ribosome

Summary of research on interaction between the ribosome and its ligands during all steps of protein synthesis in a well-characterized E. coli translation system, using cryo-EM.

Link: The Ribosome

The Ryanodine Receptor

Summary of research on the functional states of the ryanodine receptor (RyR) and 3D structure of the triad junction.

Link: The Ryanodine Receptor

Through the Microscope: Blood Cells

An internet gallery of health science images from light and electron microscopes.

Link: Through the Microscope: Blood Cells

Cell Centered Database

The Cell Centered Database (CCDB) is a web accessible database for high resolution 2D, 3D and 4D data from light and electron microscopy, including correlated imaging.

Link: Cell Centered Database

George E. Palade EM Slide Collection

The collection includes some of the earliest electron micrographs from Rockefeller University and Yale University. Many of the images are significant for their contribution to our understanding of cellular structure and processes. The images were used in the cell biology courses at Yale University by Drs. George Palade and Marilyn Farquhar. It was Dr. Palade's wish that the collection of images be freely available to students and scientists world-wide.

Link: George E. Palade EM Slide Collection

Olympus BioScapesTM Digital Imaging Competition

Olympus America Inc. is sponsoring an exciting new photo competition that will honor extraordinary microscope images of life science subjects.

Link: Olympus BioScapesTM Digital Imaging Competition

Green Fluorescent Protein

what is Green Fluorescent Protein and why is it so important.

Link: Green Fluorescent Protein

High-throughput Vertebrate Screening

a high-throughput platform for cellular resolution

Link: High-throughput Vertebrate Screening

Single-molecule recognition imaging microscopy

Describes a technique in an AFM that allows recognition of a specific type of molecule in a complex sample (chromatin) while simultaneously yielding high-resolution topographic images.

Link: Single-molecule recognition imaging microscopy

Intracellular Aspects of the Process of Protein Secretion

George E. Palade – Nobel Lecture, December 12, 1974

Link: Intracellular Aspects of the Process of Protein Secretion

Journey Into the Cell

History of electron microscopy at Rockefeller University and the

Link: Journey Into the Cell

Master´s Degree Programme in Biomedical Imaging

The Faculty of of Medicine offers from the autumn term 2010 a new master's degree programme taught in English: Master's Degree Programme in Biomedical Imaging.

Link: Master´s Degree Programme in Biomedical Imaging

Protocols for TEM specimen preparation and immnolabelling

These pages contain information on light microscopy, transmission electron microcopes, cryosectioning, immunocytochemistry and other imaging techniques used in modern biomedical research.

Link: Protocols for TEM specimen preparation and immnolabelling

Specimen Preparation in Cell Biological Applications of Fluorescence Microscopy

Link: Specimen Preparation in Cell Biological Applications of Fluorescence Microscopy