A New Approach to Fluorescence Microscopy

GE & Science Prize for Young Life Scientists winning essay by Mark Bates describing his discovery of a new type of “optically switchable” fluorescent molecule, and how these molecules were used for high-resolution biological imaging.

Link: A New Approach to Fluorescence Microscopy

Popular Mechanics:

Mehmet Fatih Yanik and his student, William Putnam, propose a non-invasive electron microscope that won't destroy living cells by using a quantum mechanical measurement technique that allows electrons to sense objects remotely.

Link: Popular Mechanics:

Shattering the diffraction limit of light: A revolution in fluorescence microscopy?

Link: Shattering the diffraction limit of light: A revolution in fluorescence microscopy?

Super-Resolution Microscopy Captures Molecules in Motion

A new twist on a sophisticated light microscopy technique is enabling researchers to capture short videos of fast-moving cellular processes while delivering super high resolution images of whole cells.

Link: Super-Resolution Microscopy Captures Molecules in Motion

A study of tissue culture cells by electron microscopy: Methods and preliminary observations.

The first electron microscope image of an intact cell at a magnification of 1600x. The image reveals mitochondria, the Golgi apparatus and a "lace-like reticulum" which was later named the "endoplasmic reticulum" by Keith Porter. The work was a collaboration between Albert Claude and Keith Porter from the Rockefeller Institute for Medical Research and Earnest Fullam of the Interchemical Corporation. the original publication is available in online.

Link: A study of tissue culture cells by electron microscopy: Methods and preliminary observations.

Cell Centered Database

The Cell Centered Database (CCDB) is a web accessible database for high resolution 2D, 3D and 4D data from light and electron microscopy, including correlated imaging.

Link: Cell Centered Database

George E. Palade EM Slide Collection

The George Pallade collection includes early electron micrographs from Rockefeller University and Yale University. Many of the images are significant for their contribution to our understanding of cellular structure and processes. The images were used in the cell biology courses at Yale University by Drs. George Palade and Marilyn Farquhar. It was Dr. Palade's wish that the collection of images be freely available to students and scientists world-wide.

Link: George E. Palade EM Slide Collection

Olympus BioScapesTM Digital Imaging Competition

Olympus America Inc. is sponsoring an exciting new photo competition that will honor extraordinary microscope images of life science subjects.

Link: Olympus BioScapesTM Digital Imaging Competition

Blackburn Gets Personal, Reflects on Path Leading Up to Nobel Prize

A week after being named UCSF’s—and her native Australia’s—first woman to receive a Nobel Prize in Physiology or Medicine, molecular biologist Elizabeth Blackburn, PhD, sat down with UCSF Chancellor Sue Desmond-Hellmann, MD, MPH, to discuss the elusive goal of work-life balance and the importance of following one’s passions and making time for “intense relaxation.”

Link: Blackburn Gets Personal, Reflects on Path Leading Up to Nobel Prize

Cell Fractionation, Biochemistry, and Electron Microscopy: The Birth of Modern Cell Biology

ert Claude pioneered techniques of cell fractionation and was the first to use an electron microscopes to study cells. This first image of an intact cell, a collaboration with Keith Portor and Ernest Fullam, was termed it the "birth certificate" of the field of cell biology by George Palade. This summary of his work at Rockefeller University includes links to selected publications.

Link: Cell Fractionation, Biochemistry, and Electron Microscopy: The Birth of Modern Cell Biology

Dr. Janet Rowley

In the 1970s, Dr. Janet Rowley identified a specific genetic “translocation” in leukemia, heralding a new understanding of the role of some translocations in specific cancers.

Link: Dr. Janet Rowley

From Intracellular Morphology to Function: The Secretory Pathway

Working in Albert Claude research group at Rockefeller University, George Palade developed the sucrose method for cell fractionation which isolated organelles without distorting their shapes. By using osmium tetroxide better contrast was obtained in electron microscopy and Palade was able to describe the fine structure of mitochondria. This summary of Palade's work at Rockefeller University include a link references to journal articles.

Link: From Intracellular Morphology to Function: The Secretory Pathway

George E. Palade The Nobel Prize in Physiology or Medicine 1974 Autobiography

This autobiography/biography was written at the time of the award and later published in the book series Les Prix Nobel/Nobel Lectures.

Link: George E. Palade The Nobel Prize in Physiology or Medicine 1974 Autobiography

George E. Palade: Legacy of a Distinguished Scientist:

In the early 1950s, Palade’s utilization of the then new technologies, such as electron microscopy and ultracentrifugation,brought understanding of the cell to a new level.

Link: George E. Palade: Legacy of a Distinguished Scientist:

George Emil Palade: How Sucrose and Electron Microscopy Led to the Birth of Cell Biology

Biography of George E. Palade and his pioneering work in cell biology and electron micrscopy. Palade used the electron microscopy to study the structures of mitochondria, the endoplasmic reticulum, and chemical synapses and to monitor cell fractionation.

From: Journal of Cell Biology
Link: George Emil Palade: How Sucrose and Electron Microscopy Led to the Birth of Cell Biology

Building a Blueprint of the Cell's Protein Factory

HHMI Bulletin, January 2001

Link: Building a Blueprint of the Cell's Protein Factory

Disease Carriers: Explore the Microscopic World of Health Science

Learn about the science underlying public health issues by perusing -- and using -- digitized images on view.

Link: Disease Carriers: Explore the Microscopic World of Health Science

Fly Light Project

Link: Fly Light Project

Mitochondria

Summary of researh using electron tomography to study the invaginations in the mitochondrial inner membran.

Link: Mitochondria

Optical Aspirations

Article on the use of cryo electron microscopy to visualize tiny three-dimensional protein structures inside cells. Nikolaus Grigorieff's lab at HHMI.

Link: Optical Aspirations

The Fly EM Project

Using electron microscopy the Fly EM project at Janelia Farm is developing technology to create a map of every neuron and synapse in the Drosophila nervous system. By establishing a map or fly ‘connectome’ that shows how all neurons in the fly brain are connected to each other, this will lead to a better understanding of brain function. Over 8,000 serial sections have been used to collect hundreds of thousands images. The project includes development of a semi-automated pipeline for serial section TEM reconstruction and exploring Scanning Electron Microscopy with Focused Ion Beam Milling (SEM-FIB) and Scanning Transmission Electron Microscopy (STEM) for improved data acquisition.

Link: The Fly EM Project

The Kinetochore

Summary of research using Electron tomography to assess kinetochore modulation of microtubule dynamics.

Link: The Kinetochore

The mechanism by which chromosomal DNA molecules are held together: entrapment within cohesin rings?

Link: The mechanism by which chromosomal DNA molecules are held together: entrapment within cohesin rings?

The Ribosome

Summary of research on interaction between the ribosome and its ligands during all steps of protein synthesis in a well-characterized E. coli translation system, using cryo-EM.

Link: The Ribosome

The Ryanodine Receptor

Summary of research on the functional states of the ryanodine receptor (RyR) and 3D structure of the triad junction.

Link: The Ryanodine Receptor

Through the Microscope: Blood Cells

An internet gallery of health science images from light and electron microscopes.

Link: Through the Microscope: Blood Cells

Green Fluorescent Protein

what is Green Fluorescent Protein and why is it so important.

Link: Green Fluorescent Protein

High-throughput Vertebrate Screening

a high-throughput platform for cellular resolution

Link: High-throughput Vertebrate Screening

Single-molecule recognition imaging microscopy

Describes a technique in an AFM that allows recognition of a specific type of molecule in a complex sample (chromatin) while simultaneously yielding high-resolution topographic images.

Link: Single-molecule recognition imaging microscopy

Intracellular Aspects of the Process of Protein Secretion

George E. Palade – Nobel Lecture, December 12, 1974

Link: Intracellular Aspects of the Process of Protein Secretion

Journey Into the Cell

History of electron microscopy at Rockefeller University and the

Link: Journey Into the Cell

The Subcellular World Revealed, 1945

Brief summary of the first electron microscope image of an intact cell that was published in 1945. The study was a collaboration between Albert Claude and Keith Porter from the Rockefeller Institute for Medical Research and Earnest Fullam of the Interchemical Corporation.

From: The Scientist
Link: The Subcellular World Revealed, 1945

Master´s Degree Programme in Biomedical Imaging

The Faculty of of Medicine offers from the autumn term 2010 a new master's degree programme taught in English: Master's Degree Programme in Biomedical Imaging.

Link: Master´s Degree Programme in Biomedical Imaging

Protocols for TEM specimen preparation and immnolabelling

These pages contain information on light microscopy, transmission electron microcopes, cryosectioning, immunocytochemistry and other imaging techniques used in modern biomedical research.

Link: Protocols for TEM specimen preparation and immnolabelling

Specimen Preparation in Cell Biological Applications of Fluorescence Microscopy

Link: Specimen Preparation in Cell Biological Applications of Fluorescence Microscopy