Electron Microscope Sample Preparation Web Resources

Microscope Manuals

The Diatome Handling and Use Manual: Technical Information

Includes information on diamond knives for cryo-ultramicrotomy, low angle diamond knives, as well as knives for light microscopy and histology.

From: DiATOME US
Link: The Diatome Handling and Use Manual: Technical Information

Protocols and Procedures

Microwave Sample Preparation

The sharing of methodology and protocols for microwave-assisted sample preparation, immunolabeling, decalcification and other applications

Link: Microwave Sample Preparation

Applications, Techniques, and Protocols

Product and technical information. The technical information includes papers MMA embedding and staining procedures.

From: Energy Beam Sciences
Link: Applications, Techniques, and Protocols

Electron Microscopy Sciences Technical Data Sheets

A collection of almost 100 information sheets for a wide variety of EM plastic resins, fixatives, and other products used in sample preparation.

From: Electron Microscopy Sciences
Link: Electron Microscopy Sciences Technical Data Sheets

Protocols for TEM specimen preparation and immnolabelling

These pages contain information on light microscopy, transmission electron microcopes, cryosectioning, immunocytochemistry and other imaging techniques used in modern biomedical research.

Link: Protocols for TEM specimen preparation and immnolabelling

Basic Preparation of Insect Specimens for SEM

Link: Basic Preparation of Insect Specimens for SEM

Laboratory Methods

Tissue fixation, staining, and embedding for transmission electron microscopy.

Link: Laboratory Methods

EM Lab manual

Laboratory manual with protocols for TEM and SEM preparation. Also includes instructions on operation of in house equipment.

Link: EM Lab manual

Technical Overviews

IHC World

Online information center for immunohistochemistry

From: IHC World
Link: IHC World

TEM Sample Preparation Guide

Preparation for Transmission Electron Microscopy (TEM) guide in English and French includes thinning, cleaving, polishing, and replicating of materials as well as ultramicrotomy and tissue staining.

Link: TEM Sample Preparation Guide