Cell Centered Database

The Cell Centered Database (CCDB) is a web accessible database for high resolution 2D, 3D and 4D data from light and electron microscopy, including correlated imaging.

Link: Cell Centered Database

Nikon Small World

The Nikon Small World competition, open to all microscopists, was created in 1975 to recognize excellence in photography through the light microscope, and to applaud the efforts of those involved with photomicrography.

Link: Nikon Small World

Olympus BioScapesTM Digital Imaging Competition

Olympus America Inc. is sponsoring an exciting new photo competition that will honor extraordinary microscope images of life science subjects.

Link: Olympus BioScapesTM Digital Imaging Competition

Multiple-photon excitation fluorescence microscopy

An introduction.

Link: Multiple-photon excitation fluorescence microscopy

Fluorescence Microscope Basics

A detailed interactive animation that illustrate the basics of imaging in the Fluorescence Microscope.

Link: Fluorescence Microscope Basics

Mission to the Inside of a Living Cell

Introduction to the use of probes to monitor dynamic events in living cells and the future for detection of cancer.

Link: Mission to the Inside of a Living Cell

Optical nanotechnologies for relevant physiological approaches to a modern biology

The group applies and develops technologies for the observation of large and complex 3D biological specimens as a function of time. The technology of choice is the light sheet-based fluorescence microscopy (LSFM), which illuminates a specimen from the side and observes it at an angle of 90°. The focal volumes of the detection system and of the light sheet overlap.

Link: Optical nanotechnologies for relevant physiological approaches to a modern biology

The mechanism by which chromosomal DNA molecules are held together: entrapment within cohesin rings?

Link: The mechanism by which chromosomal DNA molecules are held together: entrapment within cohesin rings?

Cell division drama unfolds under the microscope

Link: Cell division drama unfolds under the microscope

Mats G.L. Gustafsson, Ph.D

Mats Gustafsson is interested in creating new forms of biological light microscopy, especially modes with higher spatial resolution than is normally allowed by the diffraction limit.

Link: Mats G.L. Gustafsson, Ph.D

Blackburn Gets Personal, Reflects on Path Leading Up to Nobel Prize

A week after being named UCSF’s—and her native Australia’s—first woman to receive a Nobel Prize in Physiology or Medicine, molecular biologist Elizabeth Blackburn, PhD, sat down with UCSF Chancellor Sue Desmond-Hellmann, MD, MPH, to discuss the elusive goal of work-life balance and the importance of following one’s passions and making time for “intense relaxation.”

Link: Blackburn Gets Personal, Reflects on Path Leading Up to Nobel Prize

Open Microscopy Environment

OME (Open Microscopy Environment) develops open-source software and data format standards for the storage and manipulation of biological light microscopy data. A joint project between universities, research establishments and industry in Europe and the USA, OME has over 20 active researchers with strong links to the microscopy community.

Link: Open Microscopy Environment

Concepts in Fluorescence Microscopy

From: Nikon Instruments USA
Link: Concepts in Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy: Introduction and Applications

From: Nikon Instruments USA
Link: Total Internal Reflection Fluorescence Microscopy: Introduction and Applications

Handbook of Optical filters for Fluorescence Microscopy

From: Chroma Technology Corp.
Link: Handbook of Optical filters for Fluorescence Microscopy

Optimize Your System with the Right Filter Set

R. Kinoshita, Biophotonics International, Nov. 2002

Link: Optimize Your System with the Right Filter Set

Green Fluorescent Protein

what is Green Fluorescent Protein and why is it so important.

Link: Green Fluorescent Protein

SLM microscopy: scanless two-photon imaging and

Article on two photon imaging microscope that uses a diffractive spatial light modulator (SLM) to shape an incoming two-photon laser beam into any arbitrary light pattern.

Link: SLM microscopy: scanless two-photon imaging and

FP6 Automation Project

A novel imaging system providing high-content high-throughput multi-dimensional analysis of microscopic biological structure inside non-adherent living cells.

Link: FP6 Automation Project

Pushing the Envelope in Biological Imaging

Eric Betzig develops novel optical imaging tools in an effort to open new windows into molecular, cellular, and neurobiology.

Link: Pushing the Envelope in Biological Imaging

Single-molecule biology and Bioimaging Zhuang Research Lab

Zhuang Lab at Harvard University is developing real-time fluorescence imaging methods to track the behavior of single virus particles and of single viral genomes in live cells.

Link: Single-molecule biology and Bioimaging Zhuang Research Lab

Fluorescence microscopy with super-resolved optical sections

Alexander Egner1 and Stefan W. Hell, TRENDS in Cell Biology Vol.15 No.4 April 2005

Link: Fluorescence microscopy with super-resolved optical sections

Shattering the diffraction limit of light: A revolution in fluorescence microscopy?

Link: Shattering the diffraction limit of light: A revolution in fluorescence microscopy?

Sharper Image

An overview of the development of the the Palm

Link: Sharper Image

High Resolution Light Microscopy of Live Cells

Microscopy Today, V. Vodyanoy, June 2005

Link: High Resolution Light Microscopy of Live Cells

Squint Busters

October 2006 article on microscopes that are pushing resolution to single-molecule sharpness.

Link: Squint Busters

Blinded by the Light

Published in 2004, the article reviews advances in light microscopy including 4Pi, STED, confocal imaging, and total internal reflection fluorescence.

Link: Blinded by the Light

New Innovations for Biological Imaging and Instrumentation at HHMI

The Applied Physics and Instrumentation Group (APIG) at Janelia Farms seeks to understand bioimaging challenges and to harness new technologies for advanced imaging. Three initial projects will characterize this effort: higher resolution novel microscopy, high-throughput microscopy, and correlative microscopy.

Link: New Innovations for Biological Imaging and Instrumentation at HHMI

Super-Resolution Microscopy Captures Molecules in Motion

A new twist on a sophisticated light microscopy technique is enabling researchers to capture short videos of fast-moving cellular processes while delivering super high resolution images of whole cells.

Link: Super-Resolution Microscopy Captures Molecules in Motion

Nonlinear structured-illumination microscopy:

Technical details on structured-illumination microscopy.

Link: Nonlinear structured-illumination microscopy:

A New Approach to Fluorescence Microscopy

GE & Science Prize for Young Life Scientists winning essay by Mark Bates describing his discovery of a new type of “optically switchable” fluorescent molecule, and how these molecules were used for high-resolution biological imaging.

Link: A New Approach to Fluorescence Microscopy

Advancements in Fluorescence Proteins, Probes, and Biosensors

This webair is for the advanced live-cell researcher who is in the process of designing their experiments or scientists facing challenges in their current work that could benefit from optimized imaging.

From: PhotoMetrics, Inc.
Link: Advancements in Fluorescence Proteins, Probes, and Biosensors

The Virtual Microscope

The Virtual Microscope, which is available for free download supports functionality from electron, light, and scanning probe microscopes, datasets for these instruments, training materials to learn more about microscopy, and other related tools.

Link: The Virtual Microscope