3D and 4D Electron Tomography Web Resources

Forums and Listservers

3DEM

3-dimensional electron microscopy (3DEM) mailing list. The 3DEM list is a primary communication source among experts in the field of molecular and cellular electron microscopy.

Link: 3DEM

Image Galleries

Cell Centered Database

The Cell Centered Database (CCDB) is a web accessible database for high resolution 2D, 3D and 4D data from light and electron microscopy, including correlated imaging.

Link: Cell Centered Database

Introductions and Briefs

Electron microscopic tomography

Wadsworth Center, Albany, NY

Link: Electron microscopic tomography

Investigations

Mitochondria

Summary of researh using electron tomography to study the invaginations in the mitochondrial inner membran.

Link: Mitochondria

The Ryanodine Receptor

Summary of research on the functional states of the ryanodine receptor (RyR) and 3D structure of the triad junction.

Link: The Ryanodine Receptor

The Ribosome

Summary of research on interaction between the ribosome and its ligands during all steps of protein synthesis in a well-characterized E. coli translation system, using cryo-EM.

Link: The Ribosome

The Kinetochore

Summary of research using Electron tomography to assess kinetochore modulation of microtubule dynamics.

Link: The Kinetochore

Technical Overviews

3D-EM Laboratories

Listing of Laboratories from the 3DEM Web site

Link: 3D-EM Laboratories

4D Electron Tomography

Electron tomography provides three-dimensional (3D) imaging of noncrystalline and crystalline equilibrium structures, as well as elemental volume composition, of materials and biological specimens, including those of viruses and cells. This paper describes the development of 4D electron tomography by integrating the fourth dimension (time resolution) with the 3D spatial resolution obtained from a complete tilt series of 2D projections of an object

From: FEI Company
Link: 4D Electron Tomography

Techniques

Atomic-Resolution 3D Electron Microscopy with Dynamic Diffraction

Michael A. O'Keefe, Kenneth H. Downing, Hans-Rudolf Wenk, and Hu Meisheng, February 15, 2005. Lawrence Berkeley National Laboratory. Paper LBNL-57522.

Link: Atomic-Resolution 3D Electron Microscopy with Dynamic Diffraction

Three-dimensional reconstruction of single macromolecules

Wadsworth Center, Albany, NY

Link: Three-dimensional reconstruction of single macromolecules