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Guide to Microscopes and Microanalysis

David Scharf Photography

David Scharf is a scientist, a photographer, and an artist, specializing in Scanning Electron Microscope (S.E.M.) imagery. For over 30 years he has been an innovator in the science, technology, methodology, and photography of this uniquely fascinating form of imagery. His images define the state-of-the-art in this area of science and technology.

In the early '70s he developed techniques that permit SEM imaging of plant and animal specimens that are alive and in their "natural state". By by-passing traditional methods of killing, fixing, dehydrating, and gold coating, and manipulating the vacuum tube of the SEM, Scharf obtains images of life-forms with clarity and realism never before seen.

The scanning electron microscope images are colored using a new a system developed and patented by Scharf . This system uses multiple electron detectors. Each detector’s signal is encoded with an arbitrary color using a signal mixing system. The R. G. B. signals from the mixer are then sent to an A/D (analog to digital) converter to facilitate acquisition in digital format. The resulting color complexity and shading enhance perception of depth and interpretation of the subject’s topography.

To learn more about David Scharf Photography, visit his website at http://www.scharfphoto.com/

David Scharf images at www.microscopy.info

Mosquito Larva Hairs Micro-Wire Maze
Mosquito Larva Hairs from David Scharf Photography Micro-Wire Maze from David Scharf Photography
Ragweed Pollen Immune System Cell
Ragweed Pollen from David Scharf Photography Immune System Cell from David Scharf Photography
Jumping Spider Black Fly
Jumping Spider from David Scharf Photography  from David Scharf Photography

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