Sample Preparation for Electron Microscopy

Specimen Preparation

Electron microscopy of cells: a new beginning for a new century.

Improvements in both methods and instrumentation for EM are now allowing the structure of organelles and cellular subsystems to be characterized with unprecedented detail and reliability. J. Cell Biol. 153, F25-F32. McIntosh, J.R. (2001).

Protocols for TEM Specimen Preparation

Theoretical, technical, and practical explanations on how to examine biological specimens with microscopes. Includes negative staining, embedding, colloidal gold production, immunocytochemistry, crossectioning and more. From Ahmanson Center for Advanced Electron Microscopy and Imaging

Sample Preparation for Electron Cryo-Microscopy

TEM grid preparation for cryo electron microscopy. From the Timothy S. Baker Laboratory, Purdue University.

Specimen preparation for electron microscopy

Division of Veterinary Pathology, University of Bristol

Successful Electron Microscopy Immunolabeling

Contributions from Paul Webster, Michael Reiner, and JoAnne Buchanan.

Excerpts from Wood and Ellis

Sections on histology, immunohistology, staiing, fixatives, MSDS. By Roy C. Ellis

TEM Sample Preparation

More links to online resources.

Techniques in Biological Electron Microscopy

Prepartion lecture notes from Dr. Roger C. Wagner

EMBO Practical Course on Electron Microscopy, Immunocytochemistry and Stereology for Cell Biology

Sept. 2004,

Energy Beam Sciences

Product and technical information. The technical information includes papers MMA embedding and staining procedures.

Ultramicrotomy

The Diatome Handling and Use Manual: Technical Information

Includes information on diamond knives for cryo-ultramicrotomy, low angle diamond knives, as well as knives for light microscopy and histology. Diatome Diamond Knives

Tips, Tricks, and Data Sheets

Tips and tricks from the Microscopy Listserver

The site has tips and tricks for biological microscopy. Many of the postings are from the microscopy listserver. Anyone can submit a tip or trick. Please contact Greg Erdos for further information at gwe@biotech.ufl.edu
ICBR EM Core Lab, University of Florida

Electron Microscopy Science's Technical Data Sheets

A collection of almost 100 information sheets for a wide variety of EM plastic resins, fixatives, and other products used in sample preparation.